Initial and filtered de novo variant counts from whole genome sequencing.

Summary table of initial variant counts, de novo variant counts, and filtered SNVs and Indels. Treatment Groups: Cas9 only (without gRNA), no injection (uninjected embryos), sham injection (water only), Tyr2R treated (Tyr2R gRNA + Cas9), Tyr2F treated (Tyr2F gRNA + Cas9). Variants passing basic depth / quality filters: bcftools joint call variant count per animal passing joint-depth and genotype quality filters (see Methods). Candidate de novo mutations: all candidates produced by TrioDeNovo caller. Final SNVs / Final Indels: all SNVs/Indels remaining after filtering for false positives arising from low-level mosaicism, known C57BL/6NJ & C57BL/6N variants, proximity to UCSC repeat regions and further visual inspection.