Microarray expression profile of long noncoding RNAs in germinal center-like diffuse large B-cell lymphoma cell lines.

The contributions of long noncoding RNAs (lncRNAs) to the development of germinal center (GCB)-like diffuse large B-cell lymphoma (DLBCL) remain largely unknown. The aim of this study was to investigate the expression profile of lncRNAs in human GCB DLBCL cell lines (OCI-ly1 and OCI-ly19) and normal B lymphocytes by microarray. We demonstrated that 21,539 lncRNAs were expressed in all samples analyzed, of which 1,648 lncRNAs were upregulated and 2,671 lncRNAs were downregulated in GCB DLBCL cell lines (OCI-ly1 and OCI-ly19) (≥2.0-fold, P<0.05). Three groups of samples were used for microarray, and each group contains three replicates. Human GCB DLBCL cell lines (OCI-ly1 and OCI-ly19) were selected as tumor groups, and CD19-positive B cells were selected as control group. OCI-ly1 and OCI-ly19 cell lines were cultured in Dulbecco's Modified Eagle Medium (DMEM) of high glucose with 10% fetal bovine serum (FBS) at 37°C in a humidified incubator with 5% CO2. CD19-positive B cells were isolated from buffy coats of healthy donors’ blood obtained from the Regional Blood Donor Center using the human CD19 microbeads.