Replication Data for: Selective HDAC4 Inhibition by SP1-PTD Promotes Odontoblast Differentiation

This dataset comprises supplementary figures that support experimental validation steps in this study: (1) a pilot study performed to establish the optimal concentration range of SP1-PTD for inducing mineralization in MDPC23 cells, (2) flow cytometric verification of the mesenchymal stem cell phenotype in primary human dental pulp cells (HDPCs), and (3) confirmation of the absence of mycoplasma contamination in cell cultures to ensure experimental reliability. Supplementary Figure S1. Pilot study on the dose-dependent effects of SP1-PTD in MDPC23 cells. MDPC23 cells were cultured in differentiation medium containing increasing concentrations of SP1-PTD (0–50 μM) for 12 days, followed by Alizarin Red S staining to assess mineralization. Representative results are shown. Supplementary Figure S2. Verification of MSC phenotype in HDPCs using flow cytometry. Cells were characterized by flow cytometry to assess the expression of mesenchymal stem cell markers (CD73, CD90, and CD105) and the absence of hematopoietic markers (CD34 and CD45). Briefly, cells were harvested, washed, and incubated with the appropriate fluorophore-conjugated antibodies, followed by analysis on a flow cytometer (BD Accuri C6) according to the manufacturer’s instructions. Representative flow cytometry profiles are shown. Pos., positive; Neg., negative. Supplementary Figure S3. Detection of mycoplasma contamination using the MycoStrip™ assay (InvivoGen). Mycoplasma testing was performed according to the manufacturer’s protocol. A single band indicated a negative result (mycoplasma-free), whereas two bands indicated a positive result (mycoplasma contamination). Representative results are shown: left, positive control (provided by the manufacturer); middle, PBS and FBS (negative control); right, HDPCs. (2025-10-21)