Abnormal neutrophil signature in the blood and pancreas of pre-symptomatic and symptomatic type 1 diabetes

Analysis of neutrophils purified from peripheral blood of patients with symptomatic and pre-symptomatic type 1 diabetes (T1D), at risk of T1D, and healthy controls. Overall design: Subjects were classified into four groups based on type 1 diabetes (T1D) risk or diagnosis. Healthy control subjects with no family history of T1D (N=16) were donors undergoing surgery. Patients with family history of T1D were enrolled in the Type 1 Diabetes TrialNet Pathway to Prevention Trial; these subjects were divided into pre-symptomatic T1D (N=8) or at risk of T1D (N=13) based on the presence/absence (respectively) of 2 or more T1D-related autoantibodies. Symptomatic T1D subjects had clinical diagnosis of T1D, with first insulin injection no more than 10 days prior to the blood draw.