The role and mechanism of R-spondin3 in pulmonary vascular barrier dysfunction induced by mechanical ventilation

To investigate the effect of lowl tidal volume (LV) ventilation on the pathological development of severe lung injury, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to influence LV-induced lung injury. A mouse model of septic lung injury induced by cecal ligation and puncture was conducted, 24 h post-CLP,mice were exposed to LV (6ml/kg) for 12h To investigate the effect of small tidal volume ventilation on the pathological development of severe lung injury,we used a mouse model of septic lung injury induced by cecal ligation and puncture to observe the dynamic changes of lung pathology after small tidal volume ventilation for 12 h