Sequences of promoters, with transcriptional and translational (ATG) start sites shown capitalised, and the predicted early promoter core sequences underlined [19].

aThe italicised ‘t’ in F11L was mutated during recombineering to identity with vaccinia virus Western Reserve strain (VACV-WR).bThe second (downstream) ATG was used in the recombinant viruses described in the text. This is the ATG of the ORF as originally annotated in MVA [43]. The upstream ATG, however, likely represents the authentic translational start site in vaccinia virus, encoding a protein that is severely truncated in MVA by a small deletion (see text).cConventional insertion of promoter linked to ORF at the TK locus of MVA. Dashes indicate appendage of multiple cloning site (MCS).