Expression data from livers of wild-type and HLJ1 knockout mice.

HLJ1, also known as DNAJB4, belongs to this family and was first cloned from human liver cDNA in 1998. It is also a tumor suppressor gene and has been shown to be inversely associated with tumor invasive ability in the previous research. In addition, HLJ1 was shown to modulate migration of cancer via interaction with the cell cytoskeleton, especially when under acidic stress. It can significantly reduce cell viability in neuro-progenitor cells. The regulatory mechanism revealed that HLJ1 can be transcriptionally upregulated through enhancer activator protein-1 (AP-1) binding to promoter Yin Yang-1 (YY-1) and co-activator p300. We used microarrays to detail the whole-genome transcriptome of gene expression underlying HLJ1 regulation or mediation and identified significant regulatory network involved in physioligical functions. We utilized extracted total RNA from livers of wild-type mice and HLJ1 knockout (by the gene targeting strategy in mouse embryonic stem cells) mice as starting material for microarray assay. Three independent mice from wild-type and HLJ1 knockout mice were used in experiments as biological repeats.