Raw data from [Ocampo J, Chereji RV, Eriksson PR, Clark DJ. Contrasting roles of the RSC and ISW1/CHD1 chromatin remodelers in RNA polymerase II elongation and termination. Genome research. 2019;29(3):407–417, and Ocampo J, Chereji RV, Eriksson PR, Clark DJ. The ISW1 and CHD1 ATP-dependent chromatin remodelers compete to set nucleosome spacing in vivo. Nucleic acids research. 2016;44(10):4625–4635] processed as indicated in material and methods .

Living organisms, including baker yeast, tightly package and separate genetic information from the rest of the cell. The DNA is wrapped around nucleosomes to achieve folding at its lowest level. The arrangement of nucleosomes along the genome modulates the access and use of genetic information, which ultimately affects the cell's functioning. Despite efforts to study nucleosome profiles, the mechanism of their collective arrangement along the genome is poorly understood. We addressed this issue by directly comparing and classifying nucleosome profiles along the genes, which are known to support necessary information for cell functioning. Our findings show that the spatial organization of nucleosomes in genes is different from other genomic regions, and this difference is actively maintained by energy-consuming factors. The regulation and compartmentalization of nucleosomal organization require the simultaneous action of local and global processes.