RNA-sequencing of mammary epithelial cells from pubertal mice following enrichment of mammary ductal tissue or terminal end buds (TEBs).

Transcriptomic profilifing of the mammary epithelial cells in pubertal ducts and TEBs. Cells were enriched from Elf5-GFP and PR-cre/YFP mouse models to obtain basal, luminal GFP/YFP+ and luminal GFP/FYP-. Overall design: Mammary luminal epithelial cell subpopulations (Lin- EpCAM+ Cd29hi basal, Lin- EpCAM+ Cd 29lo Elf5-GFP+/PR-cre/YFP+ luminal GFP/YFP+, Lin- EpCAM+ Cd29lo Elf5-GFP-/PR-cre/YFP- luminal GFP/YFP- cells) were isolated from pubertal mammary tissue microdissected for ductal tissue and TEBs and sorted by FACS then subjected to bulk RNA-sequencing (n=2).