Summary statistics from several rounds of preliminary injections of rhodaminated dextran into juvenile rudiments.

We assessed the distribution of dye within two minutes after injection. We scored results as the compartment for which staining was brightest. Instances in which more than one compartment was stained with approximately equal intensity were scored as such. In many instances dye leaked into the blastocoel, presumably through the wound from the injection needle. Therefore, if dye was clearly distributed in either vestibule, hydrocoel or intercoelomic space (or combinations thereof) as well as the blastocoel, then we did not record blastocoel staining here. However, we did score blastocoelar staining in instances in which there was no discernible dye in any compartment in addition to staining in the blastocoel. “Could not discern” refers to instances in which we could not make a confident assessment of primary dye location, based upon differential intensity of dye. B = Blastocoel; H = hydrocoel (including the lumen of primary podia); IS = intercoelomic space; V = vestibule.Summary statistics from several rounds of preliminary injections of rhodaminated dextran into juvenile rudiments.