Mapping genotypes to chromatin accessibility profiles in single cells [Genotyping GoT-ChA amplicon]

Processed scATAC-seq sequencing data from myelofibrosis patients and raw sequencing data from scATAC-seq cell mixing experiments Overall design: We performed two cell line mixing studies to determine the accuracy and cell recovery of the GoT-ChA genotyping method. Cells with distinct genotypes and cell types of origin were mixed in a 1:1 ratio and subjected to GoT-ChA. For myeloproliferative neoplasm (MPN) patients, cells were isolated and FACS-sorted with CD34+ DAPI- gating followed by processing via the GoT-ChA single cell ATAC-seq and genotyping protocol.