Whole genome sequencing for Scaleless corn snake mapping

We crossed a scaleless female corn snake (scl/scl) and a heterozygous scaled male (+/scl) to obtain homozygous and heterozygous offspring for the scaleless locus. We sequenced whole-genome libraries of the two parental DNA samples and of two DNA pools from homozygous and heterozygous offspring in order to retrieve a mutation concordant with the scaleless genotype.