QTL Analysis in Komagataella phaffii

To perform QTL analysis in wildtype isolates of Komagataella phaffii (K. phaffii), also known as Pichia pastoris. The ultimate goal was to determine genomic variants from these wildtype isolates that are beneficial in terms of improving heterologous protein secretion in K. phaffii. The purpose of the flow cytometry aspect was to estimate ploidy of our selected segregants and isolate haploids only for whole genome sequencing. Conclusion: We achieved our goal of mapping genomic elements to single nucleotide resolution that could be beneficial to the industrial performance of K. phaffii, with respect to its application as a microbial cellular platform for the secretion of heterologous proteins.