His-Biotin-His Ubiquitin was overexpressed in various fission yeast (S. pombe) genetic backgrounds to identify ubiqutinated proteins. Normalizing to bait (ubiquitin) across samples provided insight in
Quantitative proteomics has emerged as a crucial approach to identifying ubiquitinated substrates to investigate the functions of ubiquitination in cells. In this regard, although the substrate screen
Yeast cells were incubated at 25˚C, unless otherwise stated. For SILAC, cells were grown in light labeled (0.03mg/ml Lys0, 0.02mg/ml Arg0) or heavy labeled (0.03mg/ml Lys4, 0.02mg/ml Arg6) amino acid
