Bacterial-driven odorant receptor expression shifts alter olfactory behavior in the oriental fruit fly

This work reveals a previously unknown mechanism by which gut microbiota modulate insect sensory ecology, demonstrating that Enterobacter sp. MU-1, a gut symbiont of the invasive pest Bactrocera dorsalis, reprograms host olfactory behavior through specific suppression of odorant receptor OR67c. Our integrated approach combining behavioral assays, transcriptomics, and functional validation establishes a novel paradigm of tripartite interplay between gut microbiota, chemoreceptor tuning, and behavioral adaptation. Key findings include: MU-1 colonization converts avoidance behavior to attraction in axenic flies (P < 0.01, Figure 1), while RNAi-mediated knockdown of OR67c reverses this behavioral shift (55% gene downregulation, P < 0.05, Figure 4). Temporal qPCR analysis confirms that MU-1 specifically suppresses OR67c expression (FPKM > 2-fold change in antibiotic-treated vs. recolonized flies, Figure 3), with no equivalent effects on other olfactory receptors. This microbiota-che..., , # Bacterial-driven odorant receptor expression shifts alter olfactory behavior in the oriental fruit fly

Dataset DOI: [10.5061/dryad.jdfn2z3qh](https://doi.org/10.5061/dryad.jdfn2z3qh)

## Description of the data and file structure

We have submitted all the raw data used for plotting. All the content mentioned is included in one Excel file. Each sheet in the file corresponds to the data used for each figure in the original article.

### Files and variables

#### File: Data_for_publication.xlsx

**Description:** Data for Figure 1A:Y-tube olfactory behavior of *B. dorsalis*. The numbers in the table represent the number of insects that made the corresponding choices.

Data for Figure 1B:Behavioral analysis in a T-shaped tube.The numbers in the table indicate the quantity of insects that made the corresponding choices, and the calculation of the selection index is also included in the table.

Data for Figure 2:The data on larval body length and weight. The numbers in the second row repr...,