Crosslinking and Analysis of cDNA (CRAC) of RNAs bound by Xrn1 and its tail domain

S.cerevisiae cells carrying either HTP tagged Xrn1 or its tail domain was UV crosslinked and tandemly purified and bound RNA was sent to next generation sequencing. This study was aimed to identify the RNAs bound by the Xrn1 and tail domain. We identified tail as a domain that bind RNA which is also bound by the Xrn1 active site. Thus finding the RNAs that bind tail compared to Xrn1 will tell us if the two repertoire are the same.