METHOD OF PREPARING PLANT-DERIVED VLPS
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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资源简介：
Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant， or plant matter comprising apoplast-localized VLPs， producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter， and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively， VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction， and the plant-derived VLPs are recovered from the filtered fraction. Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant， or plant matter comprising apoplast-localized VLPs， producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter， and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively， VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction， and the plant- derived VLPs are recovered from the filtered fraction.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
