Glycine max SNAP RNAseq and ChIPseq

Legumes can utilize atmospheric nitrogen (N) via symbiotic nitrogen fixation (SNF), which benefits crop production and sustainable agriculture. However, high soil inorganic N can suppress the initiation, growth and function of nodules. So far, how mature nodules respond to high N exposure is poorly understood at the molecular level. We show using co-expression network analysis of transcriptomic data in soybean that high N inhibition of nitrogenase activity and acceleration of senescence is dynamic and reversible. Our study demonstrates that SNAP TFs orchestrate a hierarchical transcriptional cascade in the N-induced transcriptional network in mature nodules.