Identification of chromatin states in mouse testicular germ cells using CUT&Tag

Here we describe successful implementation of CUT&Tag sequencing technology for profiling of protein-DNA interactions in mouse testicular germ cells. By performing CUT&Tag sequencing using antibodies against XAP5/XAP5L, we identified numerous common binding sites for both proteins. This work highlights the significant overlap in the genomic targets of XAP5 and XAP5L. Overall design: CUT&Tag experiments were performed in postnatal day 16 (P16) mouse testicular germ cells for XAP5, and in P56 mouse testicular germ cells for XAP5L, according to the manufacturer's protocol using Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro (Vazyme).