Expression of RIT1 M90I in primary cardiomyocytes

To investigate the impact of mutant RIT1 expression in cardiac cells, we isolated neonatal cardiomyocytes from mice harbouring an engineered Rit1 locus with Cre recombinase-inducible expression of the pathogenic variant Rit1M90I. Upon isolation, cardiomyocytes were treated with adenoviruses encoding for Cre recombinase to induce expression of the Rit1M90I variant. Comparative gene expression profiling analysis of RNA-seq data for primary cardiomyocytes expressing WT RIT1 or RIT1 (M90I).