Somatic PRDM2 c.4459delA mutations in colorectal cancers control histone methylation and tumor growth

Purpose: To identify the contribution of PRDM2 c.4459delA mutation to colorectal tumorigenesis Methods: We employed rAAV-mediated genome editing to correct somatic PRDM2 c.4459delA mutation in homozygously mutated cell line. Using next-generation sequencing we have compared transcriptional profile of parental and PRDM2-corrected cells. Results: RNA-seq profiling revealed that several hallmark cancer gene sets are affected by PRDM2 c.4459delA Overall design: To address if the PRDM2 wt cells were transcriptionally different at a global level compared to their parental cells that carry PRDM2 c.4459delA mutation, we performed RNA-sequencing of parental cells and three independently generated clones with corrected PRDM2. RNA-seq profile was generated for cells grown under normal culture conditions (10% FBS) as well as reduced serum conditions (0.5% FBS).