Number of F1 females screened for new over-proliferative Wolbachia variants per experimental condition.

wMelCS_b-infected G0 females, raised in control or antibiotic-treated food (12.5 μg/ml), were fed different doses of ethyl-methanesulfonate (EMS) and allowed to lay eggs in individual vials. F1 females were collected as virgins, mated to non-mutagenized males and also allowed to lay eggs individually. F1 females were used for Wolbachia titre measurement when were 10-days old. Number of F1 females tested per experimental condition is shown. (XLS)