A) Chromatins were prepared from Cdx2-inducible ES cells cultured for 48 - 60 hours in the Dox+ and Dox- conditions. Chromatin immunoprecipitation (ChIP) was carried out by using anti-FLAG M2 affinity
To investigate the genetic loci bound by MafB in the intestinal epithelium, we used CUT&RUN. Overall design: To investigate the genetic loci bound by MafB in the intestinal epithelium, we used CUT&RUN
DamID, in which a protein of interest is fused to Dam methylase, enables mapping of protein-DNA binding through readout of adenine methylation in genomic DNA. DamID offers a compelling alternative to
