Detection of <i>Batrachochytrium dendrobatidis</i> (<i>Bd</i>) in Madagascar by quantitative PCR (qPCR) and apparent prevalence including 95% confidence limits (CL) at each location as per swab results, assuming <i>Bd</i> qPCR sensitivity and specificity as per Skerratt et al. (2011) of. 729 and. 942, respectively.

Number of animals sampled, number of species represented, whether tadpoles (T) or post-metamorphic (A) animals were included, and number of tadpoles (#Tad) in the sample are reflected. Water filter results for Bd detection also presented. For sites where all amphibian swabs and water samples together tested negative for Bd, true prevalence is likely closer to the lower CL. Cumulative data for all sites sampled within the region appear in bold. *Amphibians sampled in Antananarivo but reported to have been collected from the wild in Fierenana. Detection of Batrachochytrium dendrobatidis (Bd) in Madagascar by quantitative PCR (qPCR) and apparent prevalence including 95% confidence limits (CL) at each location as per swab results, assuming Bd qPCR sensitivity and specificity as per Skerratt et al. (2011) of. 729 and. 942, respectively.