Differential gene expression of embryonic brains in maternal choline deficiency. Differential gene expression of embryonic brains in maternal choline deficiency

Choline plays an important role during pregnancy. The study focuses on gene expression profile of whole brain tissues of embryos in mouse female treated with choline deficient diet or chow diet. Overall design: Maternal choline deficiency in mouse embryos. The wild-type female was time-mated with the wild-type male. The treatment group includes normal chow diet (ND) or choline-deficient diet (CDD). Females were treated in different diet since time-mating day. Then 4 embryos per group were collected at E14.5 day old. The brains of embryos were harvested in Trizol for further RNA extraction and samples preparation for RNA-sequencing