Evaluation of intracellular amastigote replication for a selection of L. donovani field isolates.

Drug-susceptibility profile of the selected L. donovani field isolates to evaluate intracellular amastigote replication (Table A). Intracellular growth curves of the L. donovani field isolates and the reference lab strain. Using metacyclic promastigotes for infection, a decline in initial (24h) intracellular amastigote burden was observed for all the L. donovani strains tested. When ex vivo amastigotes (*) were used to infect host cells, a consistent increase in parasite burden was observed (Fig A). (DOCX)