Inhibitory effect of microRNA on carcinogenesis of colorectal cancer using CDX2P-NLS Cre;Apc+/loxP (CPC-Apc) mice (mRNA)

To observe the process of carcinogenesis, CPC-Apc mice were used as a mouse model of spontaneous colorectal cancer. MiRNAs (miR-200c, miR-302 (-a,-b,-c,-d), and miR-369 (-3p, -5p)) or negative control miRNA were intravenously injected to CPC-Apc mice from 8 weeks of age 3 times per week for 8 weeks with super carbonate apatite as drug delivery system. To know the therapeutic effect alive, endoscopic study was performed at 9 and 13 weeks. It revealed that tumor incidence was suppressed in mice with miRs compared to control. Mice were sacrificed at 15 weeks of age. Gene expression analysis of colorectal normal tissues were performed using Sure Print G3 Mouse GE 8×60K (G4852A) as one color experiments.Each sample was classified as follows; (1) normal tissue of mice injected microRNAs (n=2), (2) tumor tissue of mice injected microRNAs (n=2), (3) normal tissues of CPC-Apc mice (n=2), and (4) tumor tissues of CPC-Apc mice (n=7)