Single-cell RNA-sequencing of intratibial LLC cancer model in DMP1-Cre Mfn2-/- mice, DMP1-Cre Rhot1-/- mice, and wild-type mice.

We injected LLC1 cells into the tibia of DMP1-Cre Mfn2-/- mice, DMP1-Cre Rhot1-/- mice, and wild-type mice to study the changes in the cellular composition of the cancer microenvironment after knockout of Mfn2 or Rhot1 in osteocytes. LLC1 cells were injected intra-tibially into DMP1-Cre Mfn2-/- mice, DMP1-Cre Rhot1-/- mice, and wild-type mice. 14 days later tumor-bearing tibia were dissected and enzymatically dissociated into single cell suspension. Live cells were analyzed using scRNAseq (3 tumors from 3 mice mixed 1:1:1 for each group).