RNA-sequencing study on DSS-induced ulcerative colitis in mice of wild type and intestinal epithelial cell-sepific NCoR1 deletion.

Purpose: The goal of this study is to investigate the role of NCoR1 in protecting colon from ulcerative colitis. Overall design: Methods: NCoR1F/F (F/F) and NCoR1DIEC (DIEC ) mice were treated with water (naive) or 2.5% DSS. On day 3 post DSS treatment, RNA was prepared from colon tissue. This experiment consisted of 4-groups. 1) Water only for NCoR1F/F mice (F/F_naive). 2) DSS treated NCoR1F/F mice (F/F_DSS). 3) Water only for NCoR1DIEC mice (DIEC _naive). 4) DSS treated NCoR1DIEC mice (DIEC_DSS). RNA from three mice per group were combined as one sample, with four samples from each group subjected for RNA-seq