Comprehensive N-glycoproteomic profiling of humanized antibody expressed in transgenic mouse serum

This dataset reports a comprehensive N-glycoproteomic profiling of serum-derived humanized antibodies expressed in transgenic mice using an intact glycopeptide (IGP)–based LC–MS/MS workflow. The study aimed to identify and characterize site-specific N-glycosylation modifications of mouse serum proteins, with emphasis on antibody-related glycoforms. Following sample digestion and HILIC enrichment, glycopeptides were analyzed by high-resolution Orbitrap Astral mass spectrometry in data-dependent acquisition (DDA) mode. The identified N-linked glycopeptides were annotated for glycan composition, glycosylation heterogeneity, and functional classification through GO, KEGG, Reactome, and STRING analyses. The dataset provides an extensive resource for understanding host-dependent glycosylation patterns and their potential impact on antibody functionality.