A Method to Examine the Microarchitectures of the Decellularized Renal Tubule and Peritubular Capillary In Vivo Using Intravital Microscopy

This study contains intravital microscopy (IVM) data that examines the microarchitecture of decellularized renal tubules and peritubular capillaries using whole acellular kidney scaffolds. These acellular scaffolds are cell-free collagen-based matrices derived from native organs, which can be used as templates for regenerative medicine applications. This data set contains <i>in vivo</i> assays that evaluated the effectiveness of decellularization and structural and functional integrity of these acellular nephron compartments in the post-transplantation environment. Qualitative and quantitative assessments of scaffold DNA concentrations, tissue fluorescence signals, and structural and functional integrities of decellularized tubular and peritubular capillary segments were acquired and compared to the native (non-transplanted) organ. Cohorts of 2-3-month-old male Sprague Dawley rats were used: non-transplanted (n = 4), transplanted day 0 (n = 4), transplanted day 1 (n = 4), transplanted day 2 (n = 4), and transplanted day 7 (n = 4). OIF formatted files illustrate IVM measurement processes and are made publicly available in a data repository. Such data is intended to support scientific reproducibility, complement future renal studies, and extend the use of this powerful imaging application to analyze other organ scaffold systems.