Next Generation Sequencing Facilitates Quantitative Analysis of db/m and db/db bone marrow derived LSK (Lineage negative Sca1-ckit positive cells) Transcriptomes II

The Lin-Sca1+c-Kit+ (LSK) fraction of the bone marrow (BM) comprises multipotent hematopoietic stem cells (HSCs), which are vital to tissue homeostasis and vascular repair. While diabetes affects HSC homeostasis overall, the molecular signature of transcriptome under the conditions of long-standing type 2 diabetes (T2D;>6 months) remains unexplored. We employed the strength of Next generation sequencing to to study the differential pattern of LSK transcriptome under chronic diabetes. Micro RNA sequencing on LSK cells isolated from the bone marrow of db/m and db/db mice (6 months diabetic).