lncSMaRT translational regulation of Spire1, a new player in muscle differentiation. lncSMaRT translational regulation of Spire1, a new player in muscle differentiation

Total RNA of DHX36 RIP experiment in Human differentiating myoblasts at DM1 was extracted and subjected to unstranded paired end library prep and sequencing. DHX36 is an ATP-dependent helicase of the DEAH family that is known to be involved in unwinding of G-Quadruplex structures, regulating mRNA metabolism and translation. We demonstrated that mouse DHX36 and the lncRNA lnc-SMaRT are involved in muscle differentiation by translationally regulating G-Quadruplex containing mRNAs (Mlx and Spire1). Here, we characterize the human DHX36 interactome in differentiating myoblasts in order to find out a lnc-SMaRT analogous gene. Overall design: INPUT, DHX36 IP and IgG samples were collected from human myoblasts at day 1 of differentiation