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Polη-ChIP-seq analysis to investigate genome-wide distribution of Polη in budding yeast. Strains of interest were arrested in G2/M, followed by a site specific double strand breaks (PGAL-HO) induction for one hour. Cells were harvested before and after DSB-induction and proceed to immunoprecipitation. Polη was immunoprecipitated by the C-terminal Myc tag. Untagged strain was included as control.

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2021-09-10
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