Illumina RNA-Seq for the barley pan-transcriptome

This project contains Illumina RNA-Seq data provided by the Internal Barley Pan-Transcriptome Consortium. The 20 accessions sequenced for the first barley pan-genome project (Jayakodi et al., 2020, https://doi.org/10.1038/s41586-020-2947-8) were used for transcriptome sequencing. Five separate tissues were sampled for each accession, which encompassed embryo (including mesocotyl and seminal roots), seedling shoot, seedling root, inflorescence and caryopsis. Three biological replicates were sampled from each tissue type, amounting to 330 samples. Four samples failed quality control and were excluded. Preparation of the strand-specific dUTP RNA-Seq libraries, and Illumina paired-end 150 bp sequencing were carried out by Novogene (UK) Company Limited. Corresponding Iso-Seq data from a single pooled sample of each accession has been submitted as a separate bioproject, PRJEB64637.