FlowCytometry & CellCycle MtDNA

In Saccharomyces cerevisiae, flow cytometry enables semi-quantitative estimation of mitochondrial DNA (mtDNA) levels by measuring the difference in signals between cells lacking mtDNA and those containing it. Using flow cytometry, we investigated mtDNA accumulation in yeast cells under G1 and G2 phase cell cycle arrest conditions, employing thermosensitive mutants cdc4-3 and cdc15-1. Here, we present the source flow cytometry data associated with our manuscript. Cell cycle arrest induced a several-fold increase in mtDNA accumulation in both mutants. The total DNA levels in arrested cells correlated with cell size, suggesting a relationship between individual cell mtDNA quantity and cellular dimensions.