RNA-seq on MCF10A cells overexpressed with empty control vectors, TRAM2 and activated-YAP

We performed a CRISPR-based functional genetic screen targeting TEAD4 binding motif located within the YAP-bound enhancers. The screen identified a novel enhancer regulating TRAM2. We observed TRAM2 overexpression can induce EMT as YAP does. Then, we performed the RNA-seq to measure the transcription changes upon TRAM2 and YAP overexpression Overall design: We have three conditions including empty vector control, TRAM2-overexpression, YAP-over expression, for each sample, we have three biological replicates