Additional file 1: of Heritable components of the human fecal microbiome are associated with visceral fat

Supplementary Tables. Table S1. OTUs showing at least moderate (A >0.2) heritability in the extended TwinsUK 16S dataset. Each variance component, additive genetics (A, or heritability), shared environment (C) and unique environment (E) shown along with upper and lower 95Â % confidence intervals. Average relative abundance of the OTUs in the overall dataset is also shown. Table S2. 149 Bonferroni-significant OTU-adiposity associations. OTU-adiposity results are obtained from linear mixed effects regression models and include the original results (OTU-Adiposity) and results after adjustment for BMI (OTU-Adiposity BMI-adjusted). We also provide the OTU heritability and average OTU abundance in the cohort. The last two columns show the OTU results from the within MZ twin-pair difference analyses (MZ Diff Beta), and whether the MZ results were concordant with the linear mixed effects results. Reported phenotypes are visceral fat (VFM), subcutaneous fat (SFM), android/gynoid ratio (AGR), BMI, waist/hip ratio (WHR) and % trunk fat (pTF). Table S3. Genus-level associations with adiposity, where the collapsed taxonomy OTU genus was significantly associated with adiposity. Reported phenotypes are visceral fat (VFM), subcutaneous fat (SFM), android/gynoid ratio (AGR) and BMI. Table S4. Replication of TwinsUK (TUK-D) results in three independent cohorts, the American Gut (AG), the Flemish Gut Flora Project (FGFP) and the expanded TwinsUK dataset (TUK-R). The table lists the 97 OTUs forming 149 significant adiposity associations in TwinsUK and their association with BMI in both the replication and discovery samples. In addition, the table outlines the results of two meta-analyses that were performed across the studies, the first of just the independent cohorts, and the second including the discovery cohort. Finally, the last section of the table shows which OTUs were replicated in at least one of the replication cohorts. Table S5. Human genomic analyses at adiposity-related candidate host genes FHIT, TDRG1 and ELAVL4. The table shows significant genetic associations between the adiposity-related candidate host genetic variants and adiposity-associated OTUs. The final three columns summarize the results of the association between the host genetic variants and DNA methylation at CpG sites targeted by the Illumina 450Â k array. DNA methylation association was performed at the lead OTU-associated SNP in each locus; therefore, rs1433722 was not tested (NA). Table S6. List of the 97 BMI-associated loci reported in Locke et al. [8] that were used for analysis in this study. The last column denotes the BMI GWAS P values from Locke et al. [8]. Table S7. Demographics of the TwinsUK microbiome discovery sample and the American Gut replication sample. Table S8. ENA accession IDs for samples in this study currently available online and basic metadata. (XLSX 252 kb)