METHODS FOR CONSTRUCTING ALLOGENIC AND HIGH-ACTIVITY T-CELLS FOR IMMUNOTHERAPY

FIELD: biotechnology.SUBSTANCE: what is presented is a method for preparing T-cell(s) for immunotherapy in cancer treatment， involving genetic modification of said T-cell(s) by inactivating a gene coding an immune control point protein， and a gene which codes a T cell receptor (TCR) component; and introduction into T-cells of nucleic acid coding chimeric antigenic receptor directed against antigen associated with tumor. Also disclosed are: recovered T-cell or cell line obtained by said method; a pharmaceutical composition for treating cancer and a method of treating a patient.EFFECT: this group of inventions can find application in adaptive therapy of cancer.24 cl， 31 dwg， 15 tbl， 5 ex