Shedding of the indicated APMV strains from the upper respiratory tract of rhesus macaques.*

*Rhesus macaques in groups of four were inoculated simultaneously by the intranasal and intratracheal routes with a 1-ml inoculum per site containing 6.0 log10 PFU per ml of the indicated virus on day 0. Nasal washes were performed daily from day 0 to day 10 and on day 12.†The 50 percent egg infectious dose (EID50) virus titer (expressed as log10 EID50 per mL), determined as described in Materials and Methods. Limit of detection was 0.8 log10 per mL. For calculation of daily means, a value of 0.4 was used for samples with no detectable virus.‡For calculation of daily means, a value of 0.4 log10 per mL was used for samples with no detectable virus.§Shedding of wt APMV-5 was measured by limiting dilution assay of nasal wash fluid on Vero cells, followed by virus detection using a neuraminidase assay.