Amino acid residues at VP1-98 and VP1-145 of EV71 variants in clinical samples.

++; Positive by both virus isolation and molecular detection. +; Virus isolation negative but positive by molecular detection. ±; Virus isolation negative but positive by molecular detection with a weak signal. -; Negative by both virus isolation and molecular detection. “KE” represents deduced amino acid residues at VP1-98K and VP1-145E. “KE/EE” indicates mixed nucleotides [K(AAA) and E(GAA)] were identified at VP1-98 on sequencing chromatograms obtained from clinical samples by direct RT-PCR. n.d.; sequences could not be determined. *Sequence results were determined from the partial VP1 region obtained by the CODEHOP RT-PCR. Amino acid substitutions from the original inoculated viruses are indicated in bold. For amino acid residues in the VP1 region examined, amino acid substitutions were identified only at VP1-98 and VP1-145, except a substitution from VP1-242K to 242Q in a throat swab sample from monkey #5133 at 10 days postinfection. Amino acid residues at VP1-98 and VP1-145 of EV71 variants in clinical samples.