scRNA-seq of pancreatic islets from adult male mice with Fltp-reporter sorting and pancreatic head-tail separation

Single-cell RNA sequencing of pancreatic islets from adult male mice. To disect islet heterogeneity we sorted cells based on anatomical location and maturity marker (Fltp). Overall design: We used the Fltp reporter mouse line FltpZV (Gegg, et al., 2014; https://doi.org/10.7554/elife.03842) in male mice with the age of 4 months. Pancreas head and tail were anatomically separated prior islet isolation. Islets from six FltpZV/+ mice were pooled. Fltp Venus reporter positive and negative cells were sorted. Four libraries were generated based on anatomical region and cell sorting.