Single cell RNA sequencing time-course of mesenchymal progenitors during regeneration after notexin induced muscle injury

After tissue injury mesenchymal progenitors undergo a transient proliferative expansion, morphological transition and migration from their perivascular niche. To gain transcriptomic insights into the MP function in regeneration, a muscle-injury model was employed to activate MPs and follow their contribution to tissue regeneration. To compliment the population RNA seq profile previously obtained and to uncover the suspected heterogeneity of MPs in this context, tdTomato positive cells from selected timepoints were profiled by single cell RNA sequencing. Single cell RNA-seq analyes of enriched mesenchymal progenitors at selected timepoints during skeletal muscle regeneration.