IN Carta imaging data for BBB shuttle siRNA–Cy5 signal dynamics across major cortical cell types (NeuN+ neurons, GFAP+/SOX9+ astrocytes, IBA1+ microglia, CNP1+ oligodendrocytes) over early timepoints after systemic dosing

This dataset contains IN Carta–derived single object/cell imaging features or measurements used to quantify BBB shuttle siRNA-Cy5 signal dynamics across major cortical cell types following systemic dosing. Cell types analyzed include: NeuN+ neurons GFAP+/SOX9+ astrocytes IBA1+ microglia CNP1+ oligodendrocytes Data were generated from high-content imaging and processed to extract per-cell fluorescence intensity measurements (Cy5 channel, background-subtracted), followed by normalization to naive baseline conditions. The dataset includes: Raw IN Carta Cells_ObjectData outputs Processed per-cell measurements Per-well (animal-level) summary statistics Group-level aggregated data used for figure generation These data support analysis of temporal dynamics of BBB shuttle uptake across cell types at multiple timepoints post-dose. Data are organized by cell type and experimental group (A–K), corresponding to timepoints post-dose. Each folder contains IN Carta output files (Cells_ObjectData) used for downstream analysis.