Analysis of protein interactions with Tlp^sig_2.3.4 using the yeast two-hybrid system.

Testing for interactions of Tlpsig2.3.4 fused to the GAL-4 activation domain (AD- Tlpsig2.3.4) and GAL-4 binding domain (BD- Tlpsig2.3.4) with all chemotaxis proteins and individual domains fused to the GAL-4 DNA binding domain and GAL-4 activation domain respectively. Co-transformation of AD-CheW and BD-CheA was used as a positive control when testing for interactions, with a result of +++. +++: cream, dense growth of >75% of co-transformants observed on high stringency media and intermediate stringency media. ++: cream − pink moderate growth for ∼75% or more of co-transformants observed on high stringency media and intermediate stringency media. +: cream − pink light growth for ∼50% or more of co-transformants observed on high stringency media and/or intermediate stringency media. 0: no growth of co-transformants observed.