Tongue Swab Sequencing Results

DNA from tongue swab samples was separated by a DNA Isolation Kit (Coyote), followed by PCR amplification using IS6110 specific primers (F: CTGCCCAGGTCGACACATAG; R: ACTCAAGGAGCACATCAGCC). Then PCR products were isolated by DNA agarose gel according to molecular weight and purified by DNA Gel Purification Kit (Shuomei). When there is no band in DNA gel, the sample is judged as MTB negative. Purified DNA was sequenced using the Sanger method on an ABI 3730XL instrument and aligned with a standard IS6110 gene sequence. The lowest detection concentration threshold is 20 CFU/mL.