Patricia Wadsworth, 2007 Olympus BioScapes Digital Imaging Competition (2012) CIL:41466, Sus scrofa domestica, epithelial cell. CIL. Dataset

Confocal image of a mitotic spindle in a dividing cell. The spindle is shown in yellow and the surrounding actin cytoskeleton is in blue. Images were acquired using a 100X NA 1.4 phase objective lens and a Perkin Elmer spinning disc confocal scan head. A Hammamatsu Orca ER cooled CCD camera was used to collect images. Image collection was driven using Metamorph software. Complete Z-series of images were collected and deconvolved using AutoQuant software. The image is a maximum intensity projection of a subset of the entire Z-stack; the same subset of the Z-stack is used in each channel. Sixth Prize, 2007 Olympus BioScapes Digital Imaging Competition.

本数据集呈现了分裂细胞中具有丝状结构的图像，其中纺锤体以黄色显示，周围肌动蛋白细胞骨架则以蓝色呈现。图像系采用100倍NA 1.4相位物镜及珀金埃尔默旋转盘共聚焦扫描头采集。图像收集过程中，使用了Hamamatsu Orca ER冷却型CCD相机。图像收集由Metamorph软件驱动。通过AutoQuant软件收集了完整的Z系列图像并进行去卷积处理。该图像是对整个Z堆栈子集的最大强度投影；每个通道均使用相同的Z堆栈子集。本图像荣获2007年第六届Olympus BioScapes数字成像竞赛。