RNA-seq of Arabidopsis thaliana seedlings growing axenically or in the presence of Arthrobacter CL28, isolate Variovorax CL14, or both isolates together

This study evaluated the transcriptomic profiles of Arabidopsis thaliana (Col-0) plants grown along bacterial isolates whose interactions induce root morhology changes in the plant Overall design: All seeds were surface-sterilized with 70% bleach, 0.2% Tween-20 for 8?min, and rinsed three times with sterile distilled water to eliminate any seed-borne microbes on the seed surface. Seeds were stratified at 4 °C in the dark for two days. Plants were germinated on vertical square 10 X 10 cm agar plates with JM containing 0.5% sucrose, for 7 days. Then, 10 plants were transferred to each of the bacteria-inoculated agar plates containg JM without sucrose. RNA-seq of Arabidopsis thaliana seedlings growing axenically or in the presence of the primary root growth inhibitor (RGI) isolate Arthrobacter CL28, the RGI reverter isolate Variovorax CL14 or both isolates together