RNA-seq analysis of emf1, lhp1, ebs shl and ebs shl lhp1 transcriptomes in Arabidopsis thaliana

By RNA deep sequencing, around 11,000 genes were found to be differentially expressed in ebs shl lhp1 compared with wild type, and SHL and EBS, together with LHP1 and EMF1, co-regulate the expression of thousands of gene in Arabidopsis Total RNAs were extracted from wild type, lhp1, ebs shl, ebs shl lhp1 and emf1 3-week old seedings with three biological repeats and mRNA were deep sequencing by Hi-Seq 2500 (Illumina).