Adenovirus transducion of CV1.eSPR on in vitro differentiated human primary macrophages

Adenovirus transducion of CV1.eSPR on in vitro differentiated human primary macrophages Buffy coat was isolated from leukapheresis cones by Ficoll-Paque centrifugation. Monocytes were enriched from human PBMC by CD14 beads positive selection (Miltenyi Biotec) and cultured in IMDM supplanted with 10% human AB serum (omega scientific, Cat# HS-20) for 6 days. Cells were replated. On day7, cells were transduced with MOI=1000 CV1.eSPR carrying adenovirus. On day9, RNA were extracted.